abstract：:Mitochondrial DNA of Biomphalaria tenagophila, a mollusc intermediate host of Schistosoma mansoni in Brazil, was sequenced and characterised. The genome size found for B. tenagophila was 13,722 bp and contained 13 messenger RNAs, 22 transfer RNAs (tRNA) and two ribosomal RNAs (rRNA). In addition to sequencing, the mit...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Jannotti-Passos LK,Ruiz JC,Caldeira RL,Murta SM,Coelho PM,Carvalho OS

更新日期：2010-07-01 00:00:00

abstract：:This was a retrospective descriptive study on a series of cases of schistosomal myeloradiculopathy (SMR) and the aim was to investigate the incidence of this disease and its clinical and epidemiological characteristics in cases diagnosed at three healthcare units in Pernambuco, Brazil between 1994-2006. The data were ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Araújo KC,Silva Cda R,Santos AG,Barbosa CS,Ferrari TC

更新日期：2010-07-01 00:00:00

abstract：:Here, we observed the uptake of membrane-impermeant molecules by cercariae as they penetrate the skin and are transformed into schistosomula. We propose that membrane-impermeant molecules, Lucifer Yellow, Propidium iodide and Hoechst 33258 enter the parasite through both thenephridiopore and the surface membrane and t...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Thornhill J,Kusel J,Oliviera FA,Ribeiro F,Lima SF,Coelho PM,McVeigh P,Mattos AC

更新日期：2010-07-01 00:00:00

abstract：:The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segm...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ortiz-Princz D,Guariglia-Oropeza V,Avila M,Correnti M,Perrone M,Gutierrez B,Torres J,Megraud F,Cavazza ME

更新日期：2010-05-01 00:00:00

abstract：:Anopheles (Nyssorhynchus) pristinus Nagaki & Sallum, n. sp. of the Myzorhynchella Section is described based on morphological characters of adult females, males, fourth-instar larvae, pupae and male genitalia. Anopheles (Nyssorhynchus) antunesi Galvão & Amaral is characterized to fix its identity and distinguish it fr...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Nagaki SS,Motta Mde A,Sallum MA

更新日期：2010-05-01 00:00:00

abstract：:Brazilian studies involving entomological succession patterns in carcasses have been used to describe the necrophagous entomofauna of a determined municipality or region with forensic objectives. Following the same objectives, an ecological study with 10 calyptrate dipterans was carried out during the winter of 2007 a...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Barbosa RR,Mello-Patiu CA,Ururahy-Rodrigues A,Barbosa CG,Queiroz MM

更新日期：2010-03-01 00:00:00

abstract：:In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Rocha FF,Araujo RN,Silva LM,Gontijo NF,Pereira MH

更新日期：2010-03-01 00:00:00

abstract：:The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients e...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Fagundes A,Schubach A,Paula CC,Bogio A,Antonio Lde F,Schiavoni PB,Monteiro Vde S,Madeira Mde F,Quintella LP,Valete-Rosalino CM,Vasconcellos Ede C,Azeredo-Coutinho RB,Pacheco RS,Marzochi MC,Marzochi KB

更新日期：2010-02-01 00:00:00

abstract：:More sensitive methodologies are necessary to improve strongyloidiasis diagnosis. This study compared the sensitivities of the McMaster modified technique and polymerase chain reaction (PCR) assays, both performed in faecal samples. Lewis rats were subcutaneously infected with 4,000, 400 or 40 infective third-stage la...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Marra NM,Chiuso-Minicucci F,Machado GC,Zorzella-Pezavento SF,França TG,Ishikawa LL,Amarante AF,Sartori A,Amarante MR

更新日期：2010-02-01 00:00:00

abstract：:Feline coprolites were examined for parasites with the aim of studying ancient infections that occurred in the Patagonian region during the Holocene period. Eggs compatible to Trichuris sp., Calodium sp., Eucoleus sp., Nematodirus sp., Oesophagostomum sp. (Nematoda), Monoecocestus sp. (Cestoda) and Eimeria macusaniens...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Fugassa MH,Beltrame MO,Bayer MS,Sardella NH

更新日期：2009-12-01 00:00:00

abstract：:The current drug options for the treatment of chronic Chagas disease have not been sufficient and high hopes have been placed on the use of genomic data from the human parasite Trypanosoma cruzi to identify new drug targets and develop appropriate treatments for both acute and chronic Chagas disease. However, the lack...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Alves-Ferreira M,Guimarães AC,Capriles PV,Dardenne LE,Degrave WM

更新日期：2009-12-01 00:00:00

abstract：:The authors describe and compare the morphological and ultrastructural characteristics of eggs from the three most recent described species of the genus Rhodnius Stål, 1859, which have not previously been studied. These species are Rhodnius colombiensis (Mejia, Galvão & Jurberg 1999), Rhodnius milesi (Carcavallo, Roch...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Santos CM,Jurberg J,Galvão C,Rosa JA,Júnior WC,Barata JM,Obara MT

更新日期：2009-11-01 00:00:00

abstract：:Few studies are available on hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infection in populations living in small and medium-sized Brazilian cities. We evaluated the seroprevalence of these viruses in selected individuals from a clinic of infectology, who were referred to...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Portelinha Filho AM,Nascimento CU,Tannouri TN,Troiani C,Ascêncio EL,Bonfim R,D'Andrea LA,Prestes-Carneiro LE

更新日期：2009-11-01 00:00:00

abstract：:Toxocara cati is a common feline parasite transmitted by the ingestion of embryonated eggs, by the transmammary route or by predation of paratenic hosts harbouring third-stage larvae in their bodies. In the present study, the larval distribution of T. cati in tissues and organs of Rattus norvegicus experimentally infe...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Santos SV,Lescano SZ,Castro JM,Chieffi PP

更新日期：2009-09-01 00:00:00

abstract：:Two essential oils of Lippia alba (Mill.) N.E. Brown (Verbenacea), the carvone and citral chemotypes and 15 of their compounds were evaluated to determine cytotoxicity and antifungal activity. Cytotoxicity assays for both the citral and carvone chemotypes were carried out with tetrazolium-dye, which showed a dose-depe...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Mesa-Arango AC,Montiel-Ramos J,Zapata B,Durán C,Betancur-Galvis L,Stashenko E

更新日期：2009-09-01 00:00:00

abstract：:An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Reis RS,Almeida-Paes R,Muniz Mde M,Tavares PM,Monteiro PC,Schubach TM,Gutierrez-Galhardo MC,Zancopé-Oliveira RM

更新日期：2009-08-01 00:00:00

abstract：:Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC-->ACC (Ser-->Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation c...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Verza M,Maschmann Rde A,Silva MS,Dalla Costa ER,Ribeiro MO,Rosso F,Suffys PN,Tortoli E,Marcelli F,Zaha A,Rossetti ML

更新日期：2009-08-01 00:00:00

abstract：:This article presents an overview of the currently available drugs nifurtimox (NFX) and benznidazole (BZN) used against Trypanosoma cruzi, the aetiological agent of Chagas disease; herein we discuss their limitations along with potential alternatives with a focus on ergosterol biosynthesis inhibitors (EBI). These comp...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Urbina JA

更新日期：2009-07-01 00:00:00

abstract：:There is a general consensus that during chronic Trypanosoma cruzi infection, the host immune system induces complex processes to ensure the control of parasite growth while preserving the potential to mount and maintain a life-long controlled humoral and cellular immune response against the invading pathogen. This re...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Sathler-Avelar R,Vitelli-Avelar DM,Teixeira-Carvalho A,Martins-Filho OA

更新日期：2009-07-01 00:00:00

abstract：:Chagas heart disease (CHD) results from infection with the protozoan parasite Trypanosoma cruzi and is the leading cause of infectious myocarditis worldwide. It poses a substantial public health burden due to high morbidity and mortality. CHD is also the most serious and frequent manifestation of chronic Chagas diseas...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Rassi A Jr,Rassi A,Marin-Neto JA

更新日期：2009-07-01 00:00:00

abstract：:Living in close association with a vertebrate host and feeding on its blood requires different types of adaptations, including behavioural adjustments. Triatomines exhibit particular traits associated with the exploitation of their habitat and food sources and these traits have been the subject of intense analysis. Ma...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Lazzari CR,Lorenzo MG

更新日期：2009-07-01 00:00:00

abstract：:Medical mycology has greatly benefited from the introduction of molecular techniques. New knowledge on molecular genetics has provided both theoretical and practical frameworks, permitting important advances in our understanding of several aspects of pathogenic fungi. Considering Paracoccidioides brasiliensis in parti...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Richini-Pereira VB,Bosco Sde M,Theodoro RC,Macoris SA,Bagagli E

更新日期：2009-07-01 00:00:00

abstract：:Nontuberculous mycobacteria are ubiquitous and saprophytic organisms that have been implicated in a wide spectrum of diseases due to an increasing number of immunocompromised patients. The natural resistance of atypical mycobacteria to classical antituberculous drugs has encouraged research into new chemotherapeutic a...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Silva JL,Mesquita AR,Ximenes EA

更新日期：2009-07-01 00:00:00

abstract：:Aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (Sap) superfamilies. Several Sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus Candida. Candida albicans, Candida tropicalis, Candida...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Parra-Ortega B,Cruz-Torres H,Villa-Tanaca L,Hernández-Rodríguez C

更新日期：2009-05-01 00:00:00

abstract：:Investigation of the aetiology of viral meningitis in Brazil is most often restricted to cases that occur in the Southern and Southeastern Regions; therefore, the purpose of this study is to describe the viral meningitis cases that occurred in state of Pará, Northern Brazil, from January 2005-December 2006. The detect...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Castro CM,Oliveira DS,Macedo O,Lima MJ,Santana MB,Wanzeller AL,Silveira Ed,Gomes Mde L

更新日期：2009-05-01 00:00:00

abstract：:The aim of this study was to determine the incidence of congenital toxoplasmosis (CT) and to assess the performances of prenatal and neonatal diagnoses. From 1994-2005, in Toulouse University Hospital, France, amniocentesis was performed on 352 pregnant women who were infected during pregnancy. All women were treated ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bessières MH,Berrebi A,Cassaing S,Fillaux J,Cambus JP,Berry A,Assouline C,Ayoubi JM,Magnaval JF

更新日期：2009-03-01 00:00:00

abstract：:In 2008, we have celebrated the centenary of the discovery of Toxoplasma gondii.Although this ubiquitous protozoan can generate devastating damage in foetuses and newborns, its treatment is the only field in which we have made little progress, despite a huge body of research, and has not yet been validated. Pregnant w...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Peyron F

更新日期：2009-03-01 00:00:00

abstract：:Toxoplasma gondii has a very wide intermediate host range and is thought to be able to infect all warm blooded animals. The parasite causes a spectrum of different diseases and clinical symptoms within the intermediate hosts and following infection most animals develop adaptive humoral and cell-mediated immune respons...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Innes EA,Bartley PM,Maley S,Katzer F,Buxton D

更新日期：2009-03-01 00:00:00

abstract：:Host lipids have been implicated in the pathogenesis of Toxoplasma gondii infection. To determine if Toxoplasma infection influences the lipid status in the normal host, we assessed serum lipids of Swiss-Webster mice during infection with the BGD-1 strain (type-2) at a series of time points. Mice were bled at days zer...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Milovanović I,Vujanić M,Klun I,Bobić B,Nikolić A,Ivović V,Trbovich AM,Djurković-Djaković O

更新日期：2009-03-01 00:00:00

abstract：:This study was undertaken to investigate the prevalence of Mansonella ozzardi infection and to estimate the parasitic infection rate (PIR) in simuliid black flies in the municipality of Pauini, Amazonas, Brazil. We used thick blood films to examine 921 individuals in 35 riverine communities along the Pauini and Purus ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Medeiros JF,Py-Daniel V,Barbosa UC,Izzo TJ

更新日期：2009-02-01 00:00:00

abstract：:A study of crepuscular and night-biting mosquitoes was conducted at remote settlements along the Padauiri River, middle Negro River, state of Amazonas, Brazil. Collections were performed with human bait and a CDC-light trap on three consecutive days per month from June 2003-May 2004. In total, 1,203 h of collection we...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Suárez-Mutis MC,Fé NF,Alecrim W,Coura JR

更新日期：2009-02-01 00:00:00

abstract：:As in many areas of Brazil, the AIDS epidemic in Curitiba is relatively stable, but surveillance is important to support public policy. The molecular characteristics of HIV may be instrumental for monitoring epidemic trends. We evaluated plasma HIV-1 RNA (n = 37) from 38 cases presenting with positive serology, who we...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ferreira JL,Thomaz M,Rodrigues R,Harrad D,Oliveira CM,Oliveira CA,Batista JP,Ito TS,Brigido LF

更新日期：2008-12-01 00:00:00

abstract：:Rhodnius ecuadoriensis is the second most important vector of Chagas Disease (CD) in Ecuador. The objective of this study was to describe (and compare) the life cycle, the feeding and defecation patterns under laboratory conditions of two populations of this specie [from the provinces of Manabí (Coastal region) and Lo...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Villacís AG,Arcos-Terán L,Grijalva MJ

更新日期：2008-11-01 00:00:00

abstract：:The seroprevalence of toxoplasmosis in 832 pregnant women in Miracema, Rio de Janeiro, was determined and 75.1% (625) and 2.0% (17) were anti-Toxoplasma gondii IgG and IgM positive, respectively. Out of the 17 IgM positive pregnant women, only one had low avidity IgG corresponding to the acute phase of the infection. ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ribeiro AC,Mutis MS,Fernandes O

更新日期：2008-09-01 00:00:00

abstract：:The goals of the present study were to evaluate the kinetics of blood parasitism by examination of fresh blood, blood culture (BC) and PCR assays and their correlation with heart parasitism during two years of infection in Beagle dogs inoculated with the Be-78, Y and ABC Trypanosoma cruzi strains. Our results showed t...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Veloso VM,Guedes PM,Andrade IM,Caldas IS,Martins HR,Carneiro CM,Machado-Coelho GL,de Lana M,Galvão LM,Bahia MT,Chiari E

更新日期：2008-09-01 00:00:00

abstract：:In this study, IgA1 levels in the milk and serum of puerperae were compared and a correlation was established between the levels of this immunoglobulin and the occurrence of parasitism. Eighty-three paired milk and serum samples were obtained from puerperal and IgA1 levels were analyzed. In addition, the presence of i...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Manhani MN,Cruz JM,Ferreira DM,Abdallah VO,Souza MA

更新日期：2008-08-01 00:00:00

abstract：:Leptoconops nosopheris sp. n. (Diptera: Ceratopogonidae) is described from a blood-filled female biting midge in Early Cretaceous Burmese amber. The new species is characterized by a very elongate terminal flagellomere, elongate cerci, and an indistinct spur on the metatibia. This biting midge contained digenetic tryp...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Poinar G Jr

更新日期：2008-08-01 00:00:00

abstract：:The leishmanicidal activity of four batches of meglumine antimoniate, produced in Farmanguinhos-Fiocruz, Brazil (TAMs), was assessed and compared to Glucantime-Aventis Pharma Ltda. Using the amastigote-like in vitro model, the active concentrations of Sb v varied from 10microg/ml to 300microg/ml for L. (L.) chagasi an...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Morais-Teixeira Ed,Carvalho AS,Costa JC,Duarte SL,Mendonça JS,Boechat N,Rabello A

更新日期：2008-06-01 00:00:00

abstract：:This report describes the isolation of a Leishmania chagasi strain from a bat (Carollia perspicillata), and its identification using biological methods and molecular characterization. The parasites were isolated in an artificial culture medium from a blood sample extracted from a bat heart. The isolate was then inocul...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： De Lima H,Rodríguez N,Barrios MA,Avila A,Cañizales I,Gutiérrez S

更新日期：2008-06-01 00:00:00

abstract：:Four superoxide dismutase (SOD) activities (SOD I, II, III, and IV) have been characterized in the epimastigote form of Trypanosoma cruzi. The total extract was subjected to two successive ammonium sulphate additions between 35 and 85%, and the resulting fraction was purified using two continuous chromatography proces...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Mateo H,Marín C,Pérez-Cordón G,Sánchez-Moreno M

更新日期：2008-05-01 00:00:00